Ficoll

Peripheral blood lymphocytes were isolated from different healthy donors using Ficoll gradients.

PLoS ONE Alerts: New Articles Tsufit Gonen-Gross et al. 2010

Peripheral Blood Mononuclear cells (PBMC) were isolated with Ficoll-Hypaque (Amersham Pharmacia).

PLoS ONE Alerts: New Articles Frances Mercer et al. 2010

For HMDM isolation, freshly isolated heparinized human blood was underlaid with an equal volume of Ficoll-Paque PLUS (Amersham Biosciences, Piscataway, NJ), and centrifuged at 485×g for 25 min.

PLoS ONE Alerts: New Articles Justin L. Spinner et al. 2010

On arrival, samples were analyzed immediately for flowcytometric immunophenotyping; remaining cells were subjected to Ficoll density centrifugation and RNA was isolated from the mononuclear cells.

Naturejobs - All Jobs V H J van der Velden 2010

Human plasmacytoid dendritic cells (hu-pDCs) were directly isolated from the peripheral blood mononuclear cell (PBMC) fraction after Ficoll density separation of Buffy coats using BDCA4 (CD304) + antibody labeled magnetic beads and elution through iron-based MidimacsLS columns (MIltenyi Biotec).

PLoS ONE Alerts: New Articles Christopher R. Cotter et al. 2010

Venous blood (20 mL) was drawn and mononuclear cells separated using a Ficoll-Paque (GE Healthcare, São Paulo, Brazil) gradient.

PLoS ONE Alerts: New Articles Mariana M. Hecht et al. 2010

From these preparations peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Paque centrifugation and two PBS washes From the PBMC, erythroid progenitor cells (EPC) were generated via culture in an expansion medium containing erythropoietic growth factors

PLoS ONE Alerts: New Articles Claudia Filippone et al. 2010

Samples from 6-7 donors (4 mL each), were collected into EDTA-K3 tubes, layered onto Ficoll-Hystopaque (density 1.077, Sigma) and centrifuged at RT for 30 min at 400xg.

PLoS ONE Alerts: New Articles Charlotte Joos et al. 2010

PBMC were isolated using Ficoll gradient immediately following collection.

PLoS ONE Alerts: New Articles Vivien Béziat et al. 2010

Briefly, cells were isolated by Ficoll density gradient separation from Buffy coats (30-60 ml each) of healthy human donors (Mount Sinai Blood Center and New York Blood Center).

PLoS ONE Alerts: New Articles Christopher R. Cotter et al. 2010