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“PCRs were carried out with Advantage Taq II Polymerase (Takara Bioscience, UK), restriction enzymes were obtained from NEB (UK), ligation reactions were set-up with T4 DNA Ligase (Promega, UK) and transformed in XL-10 Gold ultracompetent cells (Stratagene, UK).”
“Helicase helps to open up the double strand, A small little primer comes to lend a helping hand DNA polymerase helps complete the chain Ligase helps to fix the nicks that remain Chorus:”
“Drujan D, Willis MS, Murphy LO, Corpina RA, et al. (2007) The E3 Ligase MuRF1 degrades myosin heavy chain protein in dexamethasone-treated skeletal muscle.”
“The first cDNA strand reaction was used for second cDNA strand synthesis with DNA Ligase, DNA Pol I, dNTPs and RNase H (Invitrogen).”
“EcoRI buffer, 0.5 µL (1000 U) T4 DNA Ligase (high concentration, NEB), 5 µL H”
“PIAS1 Co-Localizes with FOXL2 and Acts as an E3 Ligase for FOXL2 Sumoylation”
“DNA polymerase is always up to task Helicase helps to open up the double strand, A small little primer comes to lend a helping hand DNA polymerase helps complete the chain Ligase helps to fix the nicks that remain Chorus: Theres something you should learn about, that makes the world run right Its called the central dogma, and its the key to life It describes the basic building blocks for everything that lives, DNA to RNA to proteins, simple kids!”
“Citation: Park EC, Glodowski DR, Rongo C (2009) The Ubiquitin Ligase RPM-1 and the p38 MAPK PMK-3 Regulate AMPA Receptor Trafficking.”
“Schaefer H, Rongo C (2006) KEL-8 Is a Substrate Receptor for CUL3-dependent Ubiquitin Ligase That Regulates Synaptic Glutamate Receptor Turnover.”
“The Stebbins team named it NEL for Novel E3 Ligase.”
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