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Examples
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Supernatants were analysed for steady-state PGE concentrations by radio-immunoassay (RIA) as previously described
PLoS ONE Alerts: New Articles I. Martin Sheldon et al. 2010
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Preliminary experiments with bacteria in cluster 3 found no difference between the strains from diseased and unaffected animals Supernatants were collected after 24 h and analysed for steady-state PGE and IL-8 concentrations.
PLoS ONE Alerts: New Articles I. Martin Sheldon et al. 2010
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Supernatants were obtained from transfected COS-7 cells (Amaxa) cultured in DMEM 1 g glucose/L without FCS during 48 h and total proteins concentrations were measured (BC Assay, Interchim).
PLoS ONE Alerts: New Articles Virginie Renaud et al. 2010
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Supernatants were analysed for steady-state PGE concentrations by radio-immunoassay (RIA) as described above.
PLoS ONE Alerts: New Articles I. Martin Sheldon et al. 2010
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Supernatants were collected 48 h after transfection and were sterile filtered and stored at − 80°C.
PLoS ONE Alerts: New Articles Morgan A. Sammons et al. 2010
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Supernatants were collected and equal amounts of protein were loaded on SDS-PAGE.
PLoS ONE Alerts: New Articles Virginie Renaud et al. 2010
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Supernatants from stimulated cells were harvested and stored at
PLoS ONE Alerts: New Articles Susu Zughaier et al. 2010
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Supernatants were stored at − 20°C till further use.
PLoS ONE Alerts: New Articles Vidya Devi Negi et al. 2010
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Supernatants were obtained from transfected COS-7 cells (Amaxa) cultured in DMEM 1 g glucose/L without FCS during 48 h and total proteins concentrations were measured (BC Assay, Interchim). 22,5 µg of total protein were mixed with sample buffer without reducing agent and proteins were separated by SDS-PAGE
PLoS ONE Alerts: New Articles Virginie Renaud et al. 2010
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Supernatants were collected after 24 h and analysed for steady-state PGE and CXCL1 concentrations.
PLoS ONE Alerts: New Articles I. Martin Sheldon et al. 2010
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