“Subsequently, the water molecule that forms hydrogen bonds with Tyr390 and the NH of the acetamido group is displaced to a position which allows hydrolysis to complete”
“In another exochitinase S. marcescens chiA, after the substrate glycosidic bond is protonated, Asp313 which interacts with Asp311 moves to another position where it interacts with the proton donor residue Glu315, forcing the acetamido group of − 1 sugar to rotate.”
“S. marcescens chiB, it was proposed that binding of substrate causes the − 1 sugar ring to distort to a boat conformation and rotation of Asp142 towards Glu144, thus enabling hydrogen bonding between the acetamido group, Asp142, and Glu144.”
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