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The Latin word agrarius was applied historically to laws, or their partisans, favoring the division of Roman public lands among the poorer citizens.
The Catholic Encyclopedia, Volume 1: Aachen-Assize 1840-1916 1913
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Fluorescence microscopy was used to assess whether CD46 protein is expressed by A. agrarius sperm.
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Reverse transcription polymerase chain reaction (RT-PCR) was used to assess whether A. agrarius transcribe testicular CD46 mRNA.
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The acrosome status of A. agrarius sperm was calculated over time by immunocytochemistry using peanut agglutinin lectin.
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RT-PCR was supplemented with 3 '- and 5'-rapid amplification of cDNA ends to determine the complete nucleotide sequence of A. agrarius CD46.
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The larger A. agrarius CD46 transcript has an insert between exons 10 and 11 which, if translated, would result in a novel cytoplasmic tail.
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The aim of this study was to determine whether A. agrarius sperm fail to express CD46 protein and, if so, whether A. agrarius have a faster acrosome reaction than Mus.
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A. agrarius (striped field mice) belong to a different Apodemus subgenus and have pronounced promiscuity and large relative testis size.
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We show that A. agrarius has a significantly faster spontaneous acrosome reaction rate than A. sylvaticus and Mus.
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In addition, A. agrarius CD46 transcripts have an extended AU-rich 3'-untranslated region (UTR) and a truncated 5'-UTR, resulting in failure to express spermatozoal CD46 protein.
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