dATP

T4 DNA ligase buffer with 1 mM dATP (New England BioLabs [NEB]), 400 µM of each dNTP

PLoS ONE Alerts: New Articles Ceiridwen J. Edwards et al. 2010

The DNA product was then treated with T4 DNA polymerase in the presence of dATP to generate single-stranded 5′ overhangs.

PLoS ONE Alerts: New Articles Michael D. L. Suits et al. 2010

To facilitate Illumina GA adaptor ligation, a single 'A' base was added to the 3′-ends of the blunt-end repaired aurochs DNA extracts. 32 µl of purified phosphorylated blunt end-repaired aurochs extract DNA was included in a final 50 µl reaction mixture containing: 1× Klenow fragment buffer (NEB), 200 µM dATP (Invitrogen), and 15 units Klenow fragment with 3′-to-5′ exonuclease activity (NEB).

PLoS ONE Alerts: New Articles Ceiridwen J. Edwards et al. 2010

To understand the basis of Polκ's preference for insertion of A opposite 8-oxoG, we have solved the structure of Polκ in ternary complex with a template-primer presenting 8-oxoG in the active site and with dATP as the incoming nucleotide.

PLoS ONE Alerts: New Articles Rodrigo Vasquez-Del Carpio et al. 2009

Electron density maps showed clear densities for the bound DNA, incoming dATP, and the 8-oxoG lesion.

PLoS ONE Alerts: New Articles Rodrigo Vasquez-Del Carpio et al. 2009

(Invitrogen) and subsequently dATP-tailed with Taq DNA Polymerase (Invitrogen) to facilitate cloning into the pGEM-T vector (Promega).

PLoS ONE Alerts: New Articles Mark T. Romanish et al. 2009

We crystallized the Polκ catalytic core (aa 19-526) in ternary complex with a 13-nt/18-nt primer/template presenting the 8-oxoG lesion as the templating base and with dATP as the incoming nucleotide.

PLoS ONE Alerts: New Articles Rodrigo Vasquez-Del Carpio et al. 2009

Compared to its own catalytic efficiency for C incorporation opposite undamaged G, opposite 8-oxoG, M Kinetics of dCTP and dATP incorporation opposite undamaged G and 8-oxoG by Polκ.

PLoS ONE Alerts: New Articles Rodrigo Vasquez-Del Carpio et al. 2009

[α-32P] dATP using a procedure described previously

PLoS ONE Alerts: New Articles Xuefeng Chen et al. 2009

[29] - [31], and is coordinated in the basal octahedral plane by the unesterified oxygens of dATP β - and γ-phosphates and the carboxylates of Asp107 and Asp198, and at the apical positions by the α-phosphate and the main chain carbonyl of Met108.

PLoS ONE Alerts: New Articles Rodrigo Vasquez-Del Carpio et al. 2009