“C. neoformans as dominant selectable markers (such as hygromycin B), we used the”
“SureSilencing shRNA plasmids against HSP90α and β isoforms containing the hygromycin resistance gene were obtained from SABiosciences (Frederick, MD).”
“Chr. 17, chromosome 17; hyg, hygromycin resistance gene; neoSD, neo splice donor; SA neo, splice acceptor neo; puro, puromycin resistance gene.”
“ (DsRed) were split in the middle and fused to each other with intervening drug selection markers (hygromycin - or neomycin-resistance gene) franked by loxP sites.”
“The growth medium was changed to selection medium containing 100 µg/ml of hygromycin and the selection continued for about 2 weeks until medium sized colonies grew out.”
“Fortunately, RGC5 cells are sensitive to hygromycin.”
“Hygromycin was then shuttled from the linearized hygromycin marker (Clontech) to make pIRES-hyg-pcDNA3. 1z.”
“Complementation was therefore carried out with oppDA by constructing an integrating vector containing a hygromycin-resistance cassette along with a positive-selection L5 integrase cassette.”
“E. coli and 25 µg/ml for M. smegmatis; and hygromycin B, 100 µg/ml for oppA (Rv1280c) of M. tuberculosis was amplified from cosmid MTCY50 using the primer pair”
“The white, kanamycin and hygromycin-sensitive, sucrose-resistant colonies were identified, analyzed by PCR and candidate”
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