Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. A broad-spectrum antibiotic obtained from a strain of Streptomyces hygroscopicus

Etymologies

Sorry, no etymologies found.

Examples

  • C. neoformans as dominant selectable markers (such as hygromycin B), we used the

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  • SureSilencing shRNA plasmids against HSP90α and β isoforms containing the hygromycin resistance gene were obtained from SABiosciences (Frederick, MD).

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  • Chr. 17, chromosome 17; hyg, hygromycin resistance gene; neoSD, neo splice donor; SA neo, splice acceptor neo; puro, puromycin resistance gene.

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  • [10] (DsRed) were split in the middle and fused to each other with intervening drug selection markers (hygromycin - or neomycin-resistance gene) franked by loxP sites.

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  • The growth medium was changed to selection medium containing 100 µg/ml of hygromycin and the selection continued for about 2 weeks until medium sized colonies grew out.

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  • Fortunately, RGC5 cells are sensitive to hygromycin.

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  • Hygromycin was then shuttled from the linearized hygromycin marker (Clontech) to make pIRES-hyg-pcDNA3. 1z.

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  • Complementation was therefore carried out with oppDA by constructing an integrating vector containing a hygromycin-resistance cassette along with a positive-selection L5 integrase cassette.

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  • E. coli and 25 µg/ml for M. smegmatis; and hygromycin B, 100 µg/ml for oppA (Rv1280c) of M. tuberculosis was amplified from cosmid MTCY50 using the primer pair

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  • The white, kanamycin and hygromycin-sensitive, sucrose-resistant colonies were identified, analyzed by PCR and candidate

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