immunoprecipitated

Whole cell lysates were sequentially immunoprecipitated with anti-HIF-1α (IP1) and anti-bcl-2 antibodies (IP2) and then the Western blot analysis was performed using anti-HSP90 antibody.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

To address this hypothesis, the cell lysates were firstly immunoprecipitated with anti-HIF-1α antibody, then subjected to a second immunoprecipitation with anti-bcl-2 antibody, and the immunocomplexes were analyzed by Western blot analysis using antibody against HSP90 protein.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

Whole cell lysates were immunoprecipitated (IP) with anti-HIF-1α antibody and then the Western blot analysis was performed using specific anti-HSP90 and bcl-2 antibodies.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

(B, D) Whole cell lysates were immunoprecipitated (IP) with anti-HIF-1α antibody and subjected to SDS-PAGE.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

Protein extracts were immunoprecipitated (IP) with anti-HIF-1α and then

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

Nuclear (Nucl) and cytoplasmic (Cyto) protein extracts were immunoprecipitated (IP) with anti-HIF-1α or anti-bcl-2, respectively, or control antibody

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

Total protein lysates from pulse and pulse-chase assays were immunoprecipitated by

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

Whole cell lysates were immunoprecipitated (IP) with anti-bcl-2 or control (IgG) antibodies and then the Western blot analysis was performed using anti-HIF-1α and anti-bcl-2 antibodies.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

Whole cell lysates were immunoprecipitated (IP) with anti-HIF-1α antibody and then the Western blot analysis was performed using anti-Ubiquitin antibody.

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010

Whole cell lysates were immunoprecipitated with anti-HIF-1α or control (IgG) antibodies and then the

PLoS ONE Alerts: New Articles Daniela Trisciuoglio et al. 2010