Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun chemistry A compound of
ethanol with athiol group, often specifically 2-mercaptoethanol, which is used in variousorganic chemistry applications including proteindenaturation
Etymologies
from Wiktionary, Creative Commons Attribution/Share-Alike License
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Examples
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Beta mercaptoethanol is definitely not a flavor enhancer and drives away all the dinner guests.
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Protein G Sepharose (GE Healthcare, Piscataway, NJ) beads were then added to the lysate for 3 hours, collected by low-speed centrifugation, washed three times in lysis buffer and boiled in 2X SDS-PAGE sample buffer with 2% beta-mercaptoethanol for 10 minutes to release immunoprecipitated dMfn.
PLoS ONE Alerts: New Articles Angela C. Poole et al. 2010
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Stability Oxidation stability, highly sensitive to oxidation [1] General stability information, activity is lost on resolution with acidic ammonium sulfate and can be completely restored with FAD+, but not with FMN [1], highly sensitive to sulfhydryl reagents but not to chelating agents and is stabilized by high concentrations of mercaptoethanol and glycerol [1] Storage stability,
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-20 C, can be stored up to 1 month with loss about 20% of its activity in a mixture of 50% glycerol and 0. 1% 2-mercaptoethanol [1], 4 C, loses activity rapidly in absence of mercaptoethanol [1] References [1]
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-80 C, microsomal preparations containing 10% sucrose, frozen in li - quid nitrogen, stable [3], -70 C, sucrose or glycerol plus dithiothreitol stabilize during purifica - tion and storage [5], -70 C, storage of microsomes in presence of 14 mM mercaptoethanol and 15% sucrose, stable for several weeks [7] 335
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(Gibco), 2 mM L-glutamine (Gibco), 50 µM beta-mercaptoethanol (Gibco), 1 mM sodium pyruvate
PLoS ONE Alerts: New Articles Katherine Drews-Elger et al. 2009
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(protection by dithiothreitol or mercaptoethanol [28] l) [28] 2-diethylaminoethyl-2,2-diphenylvalerate
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[5] Localization microsome < 1-8, 12 > [2-9, 13] Cloning [10] (predominantly expressed in red form of plant [11]) [11] [12] 6 Stability Temperature stability 0 (addition of 14 mM mercaptoethanol, 15% sucrose, half-life 250 min [7]) [7] 10
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(stimulates [10]) [10] thiol-containing substances (e.g. mercaptoethanol, dithiothreitol or cysteamine enhance activity [4]) [4]
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Glutamax (Gibco), 0. 1\% beta-mercaptoethanol (Gibco), 1\% penicillin-streptomycin (PEST) (Invitrogen), 10\% plasmanate (Talecris), and 10 ng / mL bFgf (Invitrogen).
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