transfections

Since cells are grown and treated under standard tissue culture conditions, treatment protocols such as transfections or drug exposure times can be optimized for each set of samples individually without affecting the assay.

PLoS ONE Alerts: New Articles 2009

Specialized equipment is necessary for electroporation, however, the technique is comparable in expense to lipofection reagents when a large number of transfections are performed.

Archive 2005-10-01 2005

These elements, often termed shuttle sequences, allow propagation and selection of the plasmid within a bacterial host to generate sufficient quantities of the vector for mammalian transfections.

Archive 2005-10-01 2005

Although transient transfections usually result in the loss of plasmid gene product over a relatively short period of time (several days), stably transfected cell lines continue to produce the guest proteins on a continuous long-term basis (ranging from months to years).

Archive 2005-10-01 2005

Now I have a little time before I have to go to a talk, and I'm trying to decide if I should do another set of transfections and start another gel.

Archive 2005-10-01 2005

For each condition, more than ten cells in two to four transfections were analyzed.

Naturejobs - All Jobs Seong J An 2010

We performed three to six transfections per condition.

Naturejobs - All Jobs Seong J An 2010

Transient transfections of AGS cells were carried out using Effectene Transfection Reagent

PLoS ONE Alerts: New Articles Nadine Rohwer et al. 2010

At least 3 independent transfections were carried out and each experiment was performed in quadruplicate.

PLoS ONE Alerts: New Articles Mara Marongiu et al. 2010

We performed three to eight transfections per condition.

Naturejobs - All Jobs Seong J An 2010