Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun Plural form of
bradyzoite .
Etymologies
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Examples
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Initially, parasite cultures containing a mixture of tachyzoites and bradyzoites were screened by RT-PCR with gene-specific primers for the initiation and stop codons of TgAaaH1&2.
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B Western blot of extracts of Type III NED strain tachyzoites, induced bradyzoites (5 day) and HFF extract negative control probed with anti-rat-tyrosine hydroxylase antibody.
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Expression of SAG1 tachyzoite specific marker and BAG 1 / SAG 4 bradyzoite specific marker genes (dashed and white bars respectively) was also followed to confirm differentiation to bradyzoites.
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Indeed, TgAaaH2 expression may be strictly stage specific as tachyzoite cultures may contain a small proportion of bradyzoites from spontaneous conversion, as found in previous studies
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Expression of SAG1 tachyzoite specific marker and BAG 1 / SAG 4 bradyzoite specific marker genes (dashed and white bars respectively) was also followed to confirm differentiation to bradyzoites.
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A 1\% Agarose gel showing detection of RT-PCR products with TgAaaH 1&2 specific primers from tachyzoites and 5 day culture induced bradyzoites from NED strain parasites.
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The sequences were also found in EST libraries for tachyzoites and bradyzoites (www. eupathdb.org).
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To confirm stage conversion to bradyzoites, the RNA expression of three marker genes was also followed throughout the experiment.
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One of the genes, TgAaaH1, is constitutively expressed while the other gene, TgAaaH2, is induced during formation of the bradyzoites of the cyst stages of the life cycle.
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Expression of T. gondii tyrosine hydroxylases in tachyzoites and bradyzoites.
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