Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. Plural form of bradyzoite.

Etymologies

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Examples

  • To confirm stage conversion to bradyzoites, the RNA expression of three marker genes was also followed throughout the experiment.

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  • The sequences were also found in EST libraries for tachyzoites and bradyzoites (www. eupathdb.org).

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  • One of the genes, TgAaaH1, is constitutively expressed while the other gene, TgAaaH2, is induced during formation of the bradyzoites of the cyst stages of the life cycle.

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  • A 1\% Agarose gel showing detection of RT-PCR products with TgAaaH 1&2 specific primers from tachyzoites and 5 day culture induced bradyzoites from NED strain parasites.

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  • Expression of SAG1 tachyzoite specific marker and BAG 1 / SAG 4 bradyzoite specific marker genes (dashed and white bars respectively) was also followed to confirm differentiation to bradyzoites.

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  • Expression of T. gondii tyrosine hydroxylases in tachyzoites and bradyzoites.

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  • Indeed, TgAaaH2 expression may be strictly stage specific as tachyzoite cultures may contain a small proportion of bradyzoites from spontaneous conversion, as found in previous studies

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  • Initially, parasite cultures containing a mixture of tachyzoites and bradyzoites were screened by RT-PCR with gene-specific primers for the initiation and stop codons of TgAaaH1&2.

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  • B Western blot of extracts of Type III NED strain tachyzoites, induced bradyzoites (5 day) and HFF extract negative control probed with anti-rat-tyrosine hydroxylase antibody.

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  • Infection normally consists of an acute stage in which the rapidly growing tachyzoites infect a range of tissues, followed by a latent stage during which slowly replicating bradyzoites form tissue cysts in muscle and brain (3,4).

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