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  • DNA intercalators doxifluridine and hoechst were also reconfirmed small molecule hits that enhanced the proportion of

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  • Blocking solution was removed by washing in PBS three times 5 minutes and antibodies specific for tubulin (1: 1000 FITC conjugated - Sigma) and phosphorylated histone H3 (1: 100 Alexa647 conjugated - BD Biosciences) were added in PBS, together with 0.7 µg/ml hoechst dye 33342 (Invitrogen), and left to incubate overnight at 4°C.

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  • KARLHEINZ FUCHS, hoechst Aktiengesellschaft, Frankfurt, Federal Republic of Germany

    Chapter 1


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