Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun organic chemistry A
hexapeptide (containing the unusualamino acid statine ) that acts as a potentinhibitor ofaspartyl proteases
Etymologies
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Examples
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Tissues were homogenized in a lysis buffer consisting of 10 mM Hepes, 1% Triton X-100, and protease inhibitor cocktail, including aprotinin, leupeptin, pepstatin A, Bestatin, E-64,
PLoS ONE Alerts: New Articles Franco Folli et al. 2010
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Cells were washed 3 times in PBS, and detached from the plate surface with cell scrapers in the presence of protease inhibitors (sodium pyrophosphate, sodium fluoride, phenylmethanesulfonyl fluoride, pepstatin A, aprotinin, leupeptin, antipain, benzamidine - all from Sigma) diluted in PBS.
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Cell extracts were obtained with RIPA buffer in the presence of protease inhibitors (sodium pyrophosphate, sodium fluoride, phenylmethanesulfonyl fluoride, pepstatin A, aprotinin, leupeptin, antipain, benzamidine - all from Sigma).
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Skin samples (0.4 g) were dissected from either the mid-dorsal region or areas of frequent grooming (e.g. the neck and behind the ears) of Inv-Cldn6-CΔ196 transgenic mice and their age-matched CD1 counterparts and whole cell protein extracts were prepared by homogenizing in SDS extraction buffer (62.5 mM Tris pH 6.8, 25% glycerol, 2% SDS and 2% β-mercaptoethanol with pepstatin A and a complete mini protease inhibitor cocktail tablet [Roche]) followed by centrifugation.
PLoS ONE Alerts: New Articles Tammy-Claire Troy et al. 2009
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