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- n. A cationic polymer, hexadimethrine bromide, used to increase the efficiency of infection of certain cells with a retrovirus in cell culture.
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The next day, the media was replaced by 250 µL of filtered lentivirus stock (MOI ≫ 1) containing 6 µg/mL hexadimethrine bromide (polybrene, Sigma Aldrich) and the plates then incubated at 37°C for 48 h.
Simple cell exposition to polybrene, the transduction reagent, or even the expression of the tRNA
− / − ASM cells in the presence of 8 µg / ml polybrene (Sigma-Aldrich).
Retroviral vector supernatant (GF medium) supplemented with polybrene (8 ug / ml) was filtered and then added to the cells.
All cell lines were stably transduced by incubating 50\% confluent cell cultures with a virus-containing medium for 12 hours in presence of polybrene (8 mg / ml; Sigma, St. Louis, MO,