Beclin

Therefore, we investigated whether Beclin 1 is regulated by E2F.

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

An earlier ChIP-on-chip study suggested Beclin 1 could be an E2F target.

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

When Weinmann and colleagues performed chromatin immunoprecipitation to clone the E2F-bound chromatins, Beclin 1 promoter was identified to be one of the promoters occupied by many E2F family members

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

We therefore hypothesized that tenascin-C might regulate Beclin 1 through 14-3-3τ.

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

Using the RNAi technique we demonstrate a novel role for one of 14-3-3 isoforms, 14-3-3τ, in the regulation of Beclin 1 expression and autophagy.

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

In this report, we investigate a role for 14-3-3τ in the expression of a putative E2F target gene involved in autophagy, Beclin 1, and uncover its critical function in the regulation of autophagy induction.

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

As shown in Figure 2A-B, tenascin-C induced 14-3-3τ and Beclin 1 when compared with fibronectin control, and the induction of Beclin 1 was blocked when 14-3-3τ was depleted.

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

This observation suggests that Beclin 1 is an E2F target gene.

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

(E) The activities of E2F1 and E2F3 toward the Beclin 1 promoter were tested in T98G cells using a human Beclin 1 promoter-Luciferase (BECN1p-Luc) reporter construct as described in the

PLoS ONE Alerts: New Articles Bing Wang et al. 2010

The levels of Beclin 1 were normalized to GAPDH levels and expressed relative to the expression of the gene in the AdCMV control.

PLoS ONE Alerts: New Articles Bing Wang et al. 2010