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Examples
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Bisulfite clone sequencing was carried out in 14 human gastric samples initially.
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The NEFH promoter was not methylated in HEK293 cells and SH-SY5Y a neuroblastoma cell line (determined by Bisulfite-sequencing analysis), and high levels of NEFH were detected in these cell lines.
PLoS ONE Alerts: New Articles Myoung Sook Kim et al. 2010
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Bisulfite treatment was carried out on genomic DNA using the EZ DNA Methylation-Gold Kit® (Zymo Research, Orange, CA) according to the manufacturer's protocol with minor modifications detailed below
PLoS ONE Alerts: New Articles Victoria Gonzalo et al. 2010
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Bisulfite (BS) conversion of gDNA was performed using the EZ DNA Methylation-Gold kit (Zymo Research, Orange, CA) according to manufacturer's protocol.
PLoS ONE Alerts: New Articles Mehregan Movassagh et al. 2010
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Bisulfite sequence analysis was performed to check the methylation status in primary tumors and normal tissues, as well as cell lines.
PLoS ONE Alerts: New Articles Ian M. Smith et al. 2009
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Bisulfite-treated DNA was amplified using primers designed by MethPrimer to span areas of CpG islands in the promoter or first intron
PLoS ONE Alerts: New Articles Ian M. Smith et al. 2009
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Alleles-allele usage (imprinting) assays; Bisulfite-bisulfite treated DNA sequence assays; MSP-methylation-specific PCR assays.
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Bisulfite sequencing refers to the treatment of isolated DNA with sodium bisulfite to convert unmethylated cytosine to uracil, with PCR converting the uracil to thymidine followed by sequencing of the resultant DNA to detect DNA methylation.
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