Definitions
Sorry, no definitions found. You may find more data at ccre.
Etymologies
Sorry, no etymologies found.
Support
Help support Wordnik (and make this page ad-free) by adopting the word CCre.
Examples
-
But, cells that show DNA-recombination co-expressed NCre and CCre, since in mice expressing only either NCre or CCre no recombination could be observed.
-
The resulting fusion proteins (termed NCre and CCre) were able to complement each other thus reconstituting functional Cre recombinase in neurons and glia of a living mouse.
-
Since abundant recombination in oligodendrocytes and neurons could be detected in the PLP-NCre x PLP-CCre - and the PLP-Cre mice with the same ROSY reporter allele, we can think it highly unlikely that these cell types might have been targeted in GFAP-NCre x PLP-CCre mice, but were not observed due to poor expression of the ROSY reporter allele.
-
Transgenic co-expression of NCre and CCre and subsequent functional complementation induced a pattern of reporter gene expression as observed with intact Cre recombinase driven by the same promoters.
-
NCre - and CCre-coding sequences are placed under the control of two different promoters
-
The expression cassettes encoding NCre and CCre were cloned into well characterised transgene vectors
-
Adeno-associated viral vectors (AAV) containing NCre or CCre under the control of different promoters were injected into brains of ROSY-reporter mice and recombination was analysed by EYFP-expression.
-
Widespread Cre complementation in astrocytes, neurons and oligodendrocytes of GFAP-NCre x GFAP-CCre mice.
-
Immunohistochemistry on vibratome slices of paraformaldehyde-fixed tissue was performed using standard procedures as described Functional complementation of NCre and CCre in vitro.
-
This mouse line-to-mouse line variability is potentiated in the split-Cre system, since two mouse lines are needed for functional complementation of NCre and CCre, and could result in underestimation of complementation.
Comments
Log in or sign up to get involved in the conversation. It's quick and easy.