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Examples
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For neuronal staining, the sections were pretreated with alcohol 50% in PBS for 1 h at room temperature, then incubated with the primary antibody (NeuN, 1/1000, Chemicon) in PBS-Triton 0. 3% for 48 h.
PLoS ONE Alerts: New Articles Raphaël Serduc et al. 2010
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Apoptotic cells were detected in situ by the indirect TUNEL method using the ApopTag® Peroxidase In Situ Apoptosis Detection Kit (Chemicon, S7100, Temecula, CA) according to the manufacturer's instructions.
PLoS ONE Alerts: New Articles Donna N. Douglas et al. 2010
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Sections were then sequentially covered with the secondary antibody solution (1: 100, biotin-conjugated anti-mouse IgG or biotin-conjugated anti-rabbit, Chemicon), and extravidin peroxidase (1: 200, Sigma).
PLoS ONE Alerts: New Articles Hortensia Sanchez-Calderon et al. 2010
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Blocking buffer consisted of PBS containing either 10\% donkey serum (Chemicon) or 0. 2\%
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To visualize GABAergic neurons, we used antibodies directed against different isoforms of the GABA synthesizing enzymes, the rabbit anti-Glutamic Acid Decarboxylase 67 (GAD67, 1: 1000, Chemicon) and the rabbit anti-GAD65 (1: 1000, Chemicon).
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To visualize GABAergic neurons, we used antibodies directed against different isoforms of the GABA synthesizing enzymes, the rabbit anti-Glutamic Acid Decarboxylase 67 (GAD67, 1: 1000, Chemicon) and the rabbit anti-GAD65 (1: 1000, Chemicon).
PLoS ONE Alerts: New Articles Cecilia Gonzalez Campo et al. 2009
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Goat anti-pyruvate kinase (AB1235) was purchased from Chemicon
PLoS ONE Alerts: New Articles Katherine Drews-Elger et al. 2009
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The antibodies used were: anti-Myc (monoclonal 9E10, polyclonal N262 and A14, from Santa Cruz Biotechnology; monoclonal 3C7, Chemicon) and anti γ-tubulin
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Antibodies used for immunohistochemistry / immunofluorescence: Pro SP-C rabbit polyclonal antibody (Chemicon international), CC10 goat polyclonal antibody (Santa Cruz Biotechnology), pan-Cytokeratin rabbit polyclonal antibody (DAKO), Ki-67 mouse monoclonal antibody
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The blot was probed for NeuN (Chemicon, 1: 1000), NF-200
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