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  • The sections were mounted with DABCO (D-2522, Sigma).

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  • The dishes were washed twice with PBS, and then 2 ml of PBS supplemented with 2. 5\% (w / v) DABCO as an antifade was added to the dishes.

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  • The slides were mounted in solution of FluorSave reagent (VWR), 2%1,4-Diazabicyclo [2.2.2] octane (DABCO: SIGMA) and 10 µg/ml 4′,6-Diamidino-2-phenyindole, dilactate (DAPI: SIGMA).

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  • Subsequently, slides were mounted using glycerol-PBS with 1, 4-diazabicyclo (2, 2, 2) octane (DABCO) and 4, 6-diamidinol

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