Definitions
from The American Heritage® Dictionary of the English Language, 4th Edition
- n. An enzyme that catalyzes the hydrolysis of DNA.
from Wiktionary, Creative Commons Attribution/Share-Alike License
- n. deoxyribonuclease
from the GNU version of the Collaborative International Dictionary of English
- n. any of numerous enzymes that catalyze the breakdown of DNA into oligonucleotides or mononucleotides.
Etymologies
Examples
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To test this hypothesis, I added DNase to the crude extract in which we monitored ATP - and ubiquitin-dependent degradation of BSA, that was used as one of our model substrates.
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The contiguous primers annealing to the common exon of the B19 genome were used for both DNA and RNA detection, the latter after DNase treatment.
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Upon completion, the reaction mixtures were treated with DNase RQ1 (Promega) at
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Manufactured in clean room conditions BRAND PCR products are DNase, DNA - and
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They have discovered that the novel effect of caspase 3 in stem cells is related to its ability to activate another protein that cuts up the cell's DNA (called caspase-activated DNase) and has also traditionally been associated with programmed cell death.
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On-column DNase I digestion was performed. 100 ng of each RNA sample was retrotranscribed with QuantiTect Whole Transcriptome Kit (Qiagen) and a 50-fold dilution of cDNAs was analyzed by quantitative Real Time PCR (qRT-PCR) using an iCycler-iQ5
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CAD (or caspase-activated DNase in full) by slicing apart other proteins holding it at bay.
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DNA was removed in two steps, on-column using Qiagen RNase-free DNase and after elution using RNase-free DNase (New England Biolabs).
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RNA was protected with 5 mM EDTA prior to DNase inactivation. cDNA was generated with the Takara RNA PCR Kit (AMV) with 500 ng of RNA as template and random 9-mers.
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DNase and a protease inhibitor cocktail (Sigma-Aldrich) were added.
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