Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • proper n. A particular restriction endonuclease.

Etymologies

from Wiktionary, Creative Commons Attribution/Share-Alike License

From E. coli, as the enzyme was isolated from strains of E. coli.

Examples

  • Briefly, K562 cells were transfected with full length HLA-Cw0301 cDNA inserted into the EcoRI and HindIII sites of expression vector pcDNA3. 1 (−) (Invitrogen, Paisley, UK) and HLA-Cw*0403 cDNA ligated into the pEF6/V5-His expression vector using the TOPO TA Expression kit

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  • A, EcoRI restriction map of a region at 104 Mb on the coordinate of chromosome 14, 1.1 Mb centromeric to the

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  • A, EcoRI restriction map of a region at 99 Mb on the coordinate of chromosome 14, 6.1 Mb centromeric to the

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  • EcoRI-digested DNA from Chagas patient blood mononuclear cells.

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  • EcoRI digestions of human DNA with a kDNA-specific probe on blots of 0. 8% agarose gels.

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  • EcoRI digestions from parents 50 and 51, and their daughters 55 and 56 with VkDT, but no nDNA nor specific anti -

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  • In control experiments each PCR was conducted with a template DNA-free blank, DNA from control individuals born in the European Continent, DNA from a Chagas patient with parasitological demonstration of Southern hybridizations were performed with EcoRI digestions of test patient DNA.

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  • EcoRI-XhoI co-digested PCR products and pLentiV5-U6 (Invitrogen) were ligated together, producing a lentiviral vector that expresses the tRNA

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  • EcoRI and HindIII, then ligated into pRSETc/His_TEV plasmid as previously described

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  • EcoRI and XbaI and cloned into pBAD24, under the control of the pBAD promoter.

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