Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- initialism organic chemistry 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, a
zwitterionic buffering agent.
Etymologies
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Examples
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I also had a friend who deliberately mislabeled a few things -- not cryptically which would piss off EH&S, but flat-out incorrectly, e.g. "Potassium Chloride" when in fact it was HEPES or similar.
Don't Mess With Me Candid Engineer 2009
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For isolation of nascent transcripts, cells were lysed in HB buffer (10% glycerol, 60 mM KCl, 15 mM NaCl, 1.5 mM HEPES pH 7.9, 0.5 mM EDTA) containing 0.3 M sucrose and 0.8% NP40.
PLoS Biology: New Articles Francesca De Santa et al. 2010
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DMEM+GlutaMAX ™ - I (Gibco, Carlsbad, CA) supplemented with fetal bovine serum (FBS, 10%), HEPES
PLoS ONE Alerts: New Articles Eiron Cudaback et al. 2010
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Extracellular solution contains (mM): NaCl 137, KCl 4, CaCl2 1.8, MgCl2 1.0, HEPES 10, Glucose 11, (pH 7.4 with NaOH).
Recently Uploaded Slideshows ShiminWang 2010
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Cells were resuspended in buffer containing 250 mM sucrose, 5 mM NaN3, 2 mM EGTA, 20 mM HEPES pH 7.4, and a protease inhibitor cocktail, as described above, and homogenized using 20 strokes of a Dounce homogenizer.
PLoS ONE Alerts: New Articles Alfredo Csibi et al. 2010
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HEPES and 10% heat-inactivated fetal bovine serum.
PLoS ONE Alerts: New Articles Tai-Ho Hung et al. 2010
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You make a buffer with HEPES at a final concentration to 0. 1M.
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For extracellular recordings of spontaneous action potentials, pipettes (5-7 MΩ) were filled with (in mM), 130 NaCl, 2.5 KCl, 10 HEPES, 10 Glucose, 2 CaCl
PLoS ONE Alerts: New Articles Guillaume Osterstock et al. 2010
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HindIII-linearized pHCV (−) (5 µg) or PCR product (2 to 5 µM) in a buffer containing 80 mM HEPES-KOH, pH 7.5, 24 mM MgCl
PLoS ONE Alerts: New Articles Michel V. L�vesque et al. 2010
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For extracellular recordings of spontaneous action potentials, pipettes (5-7 MΩ) were filled with (in mM), 130 NaCl, 2.5 KCl, 10 HEPES, 10 Glucose, 2 CaCl
PLoS ONE Alerts: New Articles Guillaume Osterstock et al. 2010
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