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  • 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, a zwitterionic buffering agent.


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  • I also had a friend who deliberately mislabeled a few things -- not cryptically which would piss off EH&S, but flat-out incorrectly, e.g. "Potassium Chloride" when in fact it was HEPES or similar.

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  • You make a buffer with HEPES at a final concentration to 0. 1M.

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  • HindIII-linearized pHCV (−) (5 µg) or PCR product (2 to 5 µM) in a buffer containing 80 mM HEPES-KOH, pH 7.5, 24 mM MgCl

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  • HEPES and 10% heat-inactivated fetal bovine serum.

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  • For isolation of nascent transcripts, cells were lysed in HB buffer (10% glycerol, 60 mM KCl, 15 mM NaCl, 1.5 mM HEPES pH 7.9, 0.5 mM EDTA) containing 0.3 M sucrose and 0.8% NP40.

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  • For extracellular recordings of spontaneous action potentials, pipettes (5-7 MΩ) were filled with (in mM), 130 NaCl, 2.5 KCl, 10 HEPES, 10 Glucose, 2 CaCl

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  • Cells were resuspended in buffer containing 250 mM sucrose, 5 mM NaN3, 2 mM EGTA, 20 mM HEPES pH 7.4, and a protease inhibitor cocktail, as described above, and homogenized using 20 strokes of a Dounce homogenizer.

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  • DMEM+GlutaMAX ™ - I (Gibco, Carlsbad, CA) supplemented with fetal bovine serum (FBS, 10%), HEPES

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  • Extracellular solution contains (mM): NaCl 137, KCl 4, CaCl2 1.8, MgCl2 1.0, HEPES 10, Glucose 11, (pH 7.4 with NaOH).

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  • The methods were linear over a concentration range from 10 to 500 ng/ml, the inter-assay precision expressed as coefficient of variation was Brain and muscle samples were homogenized in 0. 5-1 ml of buffer (120 mM KCl, 20 mM HEPES, 5 mM MgCl2, 1 mM EGTA; pH 7.2) supplemented with 5 mg/ml BSA using a small ultraturrax.

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