Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License.

  • initialism organic chemistry 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, a zwitterionic buffering agent.

Etymologies

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Examples

  • I also had a friend who deliberately mislabeled a few things -- not cryptically which would piss off EH&S, but flat-out incorrectly, e.g. "Potassium Chloride" when in fact it was HEPES or similar.

    Don't Mess With Me Candid Engineer 2009

  • For isolation of nascent transcripts, cells were lysed in HB buffer (10% glycerol, 60 mM KCl, 15 mM NaCl, 1.5 mM HEPES pH 7.9, 0.5 mM EDTA) containing 0.3 M sucrose and 0.8% NP40.

    PLoS Biology: New Articles Francesca De Santa et al. 2010

  • DMEM+GlutaMAX ™ - I (Gibco, Carlsbad, CA) supplemented with fetal bovine serum (FBS, 10%), HEPES

    PLoS ONE Alerts: New Articles Eiron Cudaback et al. 2010

  • Extracellular solution contains (mM): NaCl 137, KCl 4, CaCl2 1.8, MgCl2 1.0, HEPES 10, Glucose 11, (pH 7.4 with NaOH).

    Recently Uploaded Slideshows ShiminWang 2010

  • Cells were resuspended in buffer containing 250 mM sucrose, 5 mM NaN3, 2 mM EGTA, 20 mM HEPES pH 7.4, and a protease inhibitor cocktail, as described above, and homogenized using 20 strokes of a Dounce homogenizer.

    PLoS ONE Alerts: New Articles Alfredo Csibi et al. 2010

  • HEPES and 10% heat-inactivated fetal bovine serum.

    PLoS ONE Alerts: New Articles Tai-Ho Hung et al. 2010

  • You make a buffer with HEPES at a final concentration to 0. 1M.

    Yahoo! Answers: Latest Questions 2010

  • For extracellular recordings of spontaneous action potentials, pipettes (5-7 MΩ) were filled with (in mM), 130 NaCl, 2.5 KCl, 10 HEPES, 10 Glucose, 2 CaCl

    PLoS ONE Alerts: New Articles Guillaume Osterstock et al. 2010

  • HindIII-linearized pHCV (−) (5 µg) or PCR product (2 to 5 µM) in a buffer containing 80 mM HEPES-KOH, pH 7.5, 24 mM MgCl

    PLoS ONE Alerts: New Articles Michel V. L�vesque et al. 2010

  • For extracellular recordings of spontaneous action potentials, pipettes (5-7 MΩ) were filled with (in mM), 130 NaCl, 2.5 KCl, 10 HEPES, 10 Glucose, 2 CaCl

    PLoS ONE Alerts: New Articles Guillaume Osterstock et al. 2010

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