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Examples

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  • Cells were washed twice with wash buffer (20 mM Tris-Hcl, 150 mM NaCl [pH 7.5]) and resuspended in 400 μl of lysis buffer (50 mM Hepes-KOH [pH 7.5], 150 mM NaCl, 10\% glycerol, 0. 5\% NP-40, 1 mM EDTA, 1 mM PMSF, 2 μg / ml Leupeptin, 2 μg / ml Pepstatin A).

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