Hepes

Beads were washed four times with 20 mM Hepes pH 7.4, 200 mM NaCl, 1% NP-40, and immune complexes were eluted by heating for 5 min in reducing Laemmli sample buffer.

PLoS Biology: New Articles Casper C. Hoogenraad et al. 2010

Cells were lysed in 20 mM Na Hepes pH 7.5, 100 mM NaCl, 1% TX-100, and cleared detergent lysates were incubated with GST-Rab4 or GST beads.

PLoS Biology: New Articles Casper C. Hoogenraad et al. 2010

The cells were serum-starved for 1½-2 h in Hepes medium.

PLoS ONE Alerts: New Articles Audrun Utskarpen et al. 2010

Rescues were performed using single copies of the UAS transgene and 24B-Gal4 in Embryos were collected at 25°C and washed in PBT (1X PBS and 0. 1% Triton X-100), dechorionated in 50% bleach, rinsed in PBT, fixed in 1: 1 heptane: 4% methanol free paraformaldehyde (4%PFA with 0.1 M Hepes pH 7.4) for 15 min while shaking, devitellinated in 1: 1 heptane: methanol for

PLoS ONE Alerts: New Articles Sarada Bulchand et al. 2010

They were serum-starved for 2 hours in Hepes medium, and

PLoS ONE Alerts: New Articles Audrun Utskarpen et al. 2010

The cover-slip was transferred to a chamber insert (20/20 Technology, Wilmington, NC) containing Hank's balanced salt solution (HBSS) supplemented by 15 mM Hepes, 4.5 g/L glucose, and 350 mg/mL Na

PLoS ONE Alerts: New Articles Audrun Utskarpen et al. 2010

Tissues were homogenized in a lysis buffer consisting of 10 mM Hepes, 1% Triton X-100, and protease inhibitor cocktail, including aprotinin, leupeptin, pepstatin A, Bestatin, E-64,

PLoS ONE Alerts: New Articles Franco Folli et al. 2010

Protein samples from culture wells were collected as described from microwell plates, and lysed in WB-buffer (25 mM Hepes pH 7.5, 100 mM NaCl, 5 mM EDTA pH 8.3, 0.5% Triton X-100, 20 mM β-glycerophosphate, 100 µM orthovanadate, 0.5 mM PMSF and 1 mM DTT).

PLoS ONE Alerts: New Articles Ville Härmä et al. 2010

The cells were starved in MEM supplemented by Hepes at 37°C for 4 h.

PLoS ONE Alerts: New Articles Audrun Utskarpen et al. 2010

For immunoprecipitation, the lysates were diluted 4-fold in co-immunoprecipitation buffer (2 mM Hepes pH 7.9, 20 mM NaCl, 0,01% Tween 20, 1 mM DTT, 10% glycerol) and incubated overnight with 25 µl of anti-myc (Santa Cruz) or anti-FOXL2 agarose-conjugate antibody at 4°C.

PLoS ONE Alerts: New Articles Mara Marongiu et al. 2010