Itk

Upon TCR stimulation, SLP-76 is phosphorylated and both Vav1 and Itk bind to these phosphorylated tyrosines

PLoS ONE Alerts: New Articles Rebekah R. Bartelt et al. 2009

Itk, the common link between PLC-γ1 and Vav1, has increased expression in Jurkat E6. 1 T cells

PLoS ONE Alerts: New Articles Rebekah R. Bartelt et al. 2009

SLP-76 pY128, Itk pY511, TCR α chain and CD4 antibodies were purchased from BD Pharmingen.

PLoS ONE Alerts: New Articles Rebekah R. Bartelt et al. 2009

Vav1 requires the presence of Itk to bind phosphorylated SLP-76, although it does not appear that Vav1 and Itk interact directly

PLoS ONE Alerts: New Articles Rebekah R. Bartelt et al. 2009

Shan X, Czar MJ, Bunnell SC, Liu P, Liu Y, et al. (2000) Deficiency of PTEN in Jurkat T cells causes constitutive localization of Itk to the plasma membrane and hyperresponsiveness to CD3 stimulation.

PLoS ONE Alerts: New Articles Rebekah R. Bartelt et al. 2009

Wilcox HM, Bunnell SC, Berg LJ (1997) Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity.

PLoS ONE Alerts: New Articles Rebekah R. Bartelt et al. 2009

Phosphorylated SLP-76 is essential for bringing Vav1 and Itk to the LAT signaling complex

PLoS ONE Alerts: New Articles Rebekah R. Bartelt et al. 2009

There was some variation in the Itk expression levels in various sublines of Jurkat E6. 1 T cells, with most expressing high levels of Itk

PLoS ONE Alerts: New Articles Rebekah R. Bartelt et al. 2009

(B) The immunoblots were analyzed by densitometry and the ratio of the intensity of the phosphospecific Itk band to the pan-Itk band for each cell line for three separate experiments was averaged.

PLoS ONE Alerts: New Articles Rebekah R. Bartelt et al. 2009

The site-specific phosphorylation and expression of Itk was assessed by immunoblotting using Itk pY511 (top) and pan-Itk (bottom) antibodies.

PLoS ONE Alerts: New Articles Rebekah R. Bartelt et al. 2009