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Examples
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PCRs were carried out with Advantage Taq II Polymerase (Takara Bioscience, UK), restriction enzymes were obtained from NEB (UK), ligation reactions were set-up with T4 DNA Ligase (Promega, UK) and transformed in XL-10 Gold ultracompetent cells (Stratagene, UK).
PLoS ONE Alerts: New Articles Arthur M. Talman et al. 2010
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Drujan D, Willis MS, Murphy LO, Corpina RA, et al. (2007) The E3 Ligase MuRF1 degrades myosin heavy chain protein in dexamethasone-treated skeletal muscle.
PLoS ONE Alerts: New Articles Siva K. Panguluri et al. 2010
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PCRs were carried out with Advantage Taq II Polymerase (Takara Bioscience, UK), restriction enzymes were obtained from NEB (UK), ligation reactions were set-up with T4 DNA Ligase (Promega, UK) and transformed in XL-10 Gold ultracompetent cells (Stratagene, UK).
PLoS ONE Alerts: New Articles Arthur M. Talman et al. 2010
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Helicase helps to open up the double strand, A small little primer comes to lend a helping hand DNA polymerase helps complete the chain Ligase helps to fix the nicks that remain Chorus:
WN.com - Articles related to Plants give up some deep secrets of drought resistance 2010
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Helicase helps to open up the double strand, A small little primer comes to lend a helping hand DNA polymerase helps complete the chain Ligase helps to fix the nicks that remain Chorus:
WN.com - Articles related to Plants give up some deep secrets of drought resistance 2010
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The first cDNA strand reaction was used for second cDNA strand synthesis with DNA Ligase, DNA Pol I, dNTPs and RNase H (Invitrogen).
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EcoRI buffer, 0.5 µL (1000 U) T4 DNA Ligase (high concentration, NEB), 5 µL H
PLoS ONE Alerts: New Articles Nathan A. Baird et al. 2008
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EcoRI buffer, 0.5 µL (1000 U) T4 DNA Ligase (high concentration, NEB), 5 µL H
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EcoRI buffer, 0.5 µL (1000 U) T4 DNA Ligase (high concentration, NEB), 5 µL H
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PIAS1 Co-Localizes with FOXL2 and Acts as an E3 Ligase for FOXL2 Sumoylation
PLoS ONE Alerts: New Articles Mara Marongiu et al. 2010
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