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Examples
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The resulting fusion proteins (termed NCre and CCre) were able to complement each other thus reconstituting functional Cre recombinase in neurons and glia of a living mouse.
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These fusion proteins are referred to as NCre and CCre, respectively.
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Transgenic mouse lines expressing NCre (line GCNV) and CCre (line GCCF) were crossed to ROSY reporter mice and analysed for recombination in the cerebellum (F).
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Transgenic co-expression of NCre and CCre and subsequent functional complementation induced a pattern of reporter gene expression as observed with intact Cre recombinase driven by the same promoters.
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To analyse whether split-Cre complementation induces recombination activity in vivo, we generated transgenic mice expressing NCre and CCre under the control of the mouse proteolipid protein (PLP) and the human glial fibrillary acidic protein (GFAP) promoter
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Transgenic mouse lines expressing NCre (line PCND) and CCre (line PCCR) were crossed to ROSY reporter mice and analysed for recombination with in the corpus callosum (C).
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Therefore, we also tested NCre and CCre complementation in adeno-associated virus-mediated gene transfer
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NCre - and CCre-coding sequences are placed under the control of two different promoters
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But, cells that show DNA-recombination co-expressed NCre and CCre, since in mice expressing only either NCre or CCre no recombination could be observed.
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To evaluate the potency of split-Cre, we analyzed the progeny of GFAP+ / PLP+ cells during brain development using the GFAP-NCre x PLP-CCre-mice.
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