Nrf

All today was to Debbie was an excuse to be part firf a drama, a big exciting drama that made her the centre Nrf attention with friends and their families.

two women Cole, Martina 1999

MDA-MB-231 cells were transfected with different amounts of an ENC1-Myc expression vector, along with an expression vector for HA-Nrf2.

PLoS ONE Alerts: New Articles Xiao-Jun Wang et al. 2009

Nrf2-containing pretein complexes were immunoprecipitated with anti-Nrf2 antibodies in RIPA buffer.

PLoS ONE Alerts: New Articles Xiao-Jun Wang et al. 2009

Keap1 - / - and wild-type MEF cells were transfected with plasmids for HA-Nrf2 and ENC1-Myc.

PLoS ONE Alerts: New Articles Xiao-Jun Wang et al. 2009

MDA-MB-231 cells were transfected with HA-Nrf2 and with an empty vector or ENC1-Myc.

PLoS ONE Alerts: New Articles Xiao-Jun Wang et al. 2009

An expression vector for HA-Nrf2 or HA-SMN gene was transfected into MDA-MB-231 cells, along with an empty vector or an expression vector for ENC1-Myc.

PLoS ONE Alerts: New Articles Xiao-Jun Wang et al. 2009

To further confirm that ENC1 down-regulates Nrf2 specifically, cells were transfected with different amounts of an ENC1 expression vector, along with an expression vector for HA-Nrf2, and cell lysates were subjected to immunoblot analysis.

PLoS ONE Alerts: New Articles Xiao-Jun Wang et al. 2009

To further test this notion, a similar experiment was performed with overexpressed HA-Nrf2 to saturate endogenous Keap1, and therefore minimize the effect of Keap1 on Nrf2.

PLoS ONE Alerts: New Articles Xiao-Jun Wang et al. 2009

In order to demonstrate that the ENC1-mediated down-regulation of Nrf2 is not due to an artifact from the expression vector, but rather specific in down-regulating Nrf2, a similar experiment was performed using HA-tagged survival motor neuron (HA-SMN) in place of HA-Nrf2.

PLoS ONE Alerts: New Articles Xiao-Jun Wang et al. 2009

The precipitated protein complexes were subjected to immunoblot analysis with anti-Nrf2 and anti-ENC1 antibodies.

PLoS ONE Alerts: New Articles Xiao-Jun Wang et al. 2009