Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License.

  • noun 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, used to detect β-galactosidase.

Etymologies

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Examples

  • From the colonies that appeared on the plates, a loopful of cells was taken and resuspended in Lemco broth, mixed with vortexing, serial dilutions were plated onto Lemco agar supplemented with 2% sucrose and 50 µg/ml X-gal and allowed to grow for two days.

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  • (Invitrogen, Carlsbad, CA) and plated onto agar plates with X-gal and 150 mg/mL Carbenicillin.

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  • Unfortunately, both heterozygotes and homozygotes carrying the lacZ allele did not express β-galactosidase protein or activity by immunofluorescence or X-gal staining, respectively (data not shown).

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  • This suggests that Muc16-lacZ chimeric transcripts may be very unstable, leading to insufficient production of β-galactosidase for detection by immunofluorescence and X-gal staining (data not shown).

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  • LacZ reporter: the qualitative X-gal colony-lift filter assay and quantitative

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  • LacZ reporter: X-gal colony-lift filter assay and ortho

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  • Colonies were plated on LB Kanamycin with X-gal and grown overnight.

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  • In the X-gal colony-lift filter assay, the lysed yeast cells were incubated with X-gal for three hours, and blue color developing after three-hour incubation was not considered to be indicative of positive protein: DNA interactions.

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  • LacZ reporter: X-gal colony-lift filter assay and ortho

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  • h and then plated on Lemco agar supplemented with hygromycin B, kanamycin and 50 µg/ml X-gal.

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