ZO-1

In this model, the distribution of the tight junctional protein zonula occludens protein 1 (ZO-1) was determined since no data exists on its expression during gestation.

PLoS ONE Alerts: New Articles Tim G. A. M. Wolfs et al. 2009

The cellular distribution of tight junction protein ZO-1 was shown to be underdeveloped at low GA whereas endotoxin induced chorioamnionitis prevented the maturation of tight junctions during later gestation.

PLoS ONE Alerts: New Articles Tim G. A. M. Wolfs et al. 2009

Tight junction distribution was examined by immunofluorescent staining of frozen sections (3 µm) with an antibody to Zonula Occludens protein 1 (ZO-1).

PLoS ONE Alerts: New Articles Tim G. A. M. Wolfs et al. 2009

Although ZO-1 is not the only tight junction protein responsible for paracellular barrier sealing, it plays a central role in maintenance of paracellular intestinal barrier integrity by connection of the intercellular tight junction proteins

PLoS ONE Alerts: New Articles Tim G. A. M. Wolfs et al. 2009

Immunolocalisation of ZO-1 (red) in the fetal intestine.

PLoS ONE Alerts: New Articles Tim G. A. M. Wolfs et al. 2009

At 140d GA, a normal ZO-1 distribution was detected in control animals (D) that became disrupted upon endotoxin exposure for 30d (E).

PLoS ONE Alerts: New Articles Tim G. A. M. Wolfs et al. 2009

At 125d GA, a fragmented staining pattern for ZO-1 was seen in control animals (A) or lambs exposed to endotoxin for 2 (B) or 14d (C).

PLoS ONE Alerts: New Articles Tim G. A. M. Wolfs et al. 2009

The distribution of ZO-1 was recorded at a magnification of 200x using the Metasystems Image Pro System (black and white charge-couple device camera; Metasystems, Sandhausen, Germany) mounted on a Leica DM-RE fluorescence microscope (Leica, Wetzler, Germany).

PLoS ONE Alerts: New Articles Tim G. A. M. Wolfs et al. 2009

To our knowledge, this is the first report showing that the cellular distribution of tight junction protein ZO-1 was underdeveloped at

PLoS ONE Alerts: New Articles Tim G. A. M. Wolfs et al. 2009

In near term control tissue of 140d GA, staining of ZO-1 formed a continuous band lining the mucosal epithelium, indicating maturation of the gut barrier during gestation.

PLoS ONE Alerts: New Articles Tim G. A. M. Wolfs et al. 2009