antifade

The specimens were counterstained by incubation with Hoechst 33342 (Invitrogen, CA) for 5 min at room temperature; one drop of SlowFade antifade reagent (Molecular Probes) was dispensed on the glass slide, covered with cover slip, and imaged

PLoS ONE Alerts: New Articles Mariana M. Hecht et al. 2010

Subsequently slides were stained by Phalloidin AlexaFluor®-555 conjugated (Invitrogen) for 30 min to reveal actin organization, and mounted in Prolong® Gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen) to allow nucleus localization.

PLoS ONE Alerts: New Articles Simone Pacini et al. 2010

The specimens were counterstained by incubation with Hoechst 33342 (Invitrogen, CA) for 5 min at room temperature; one drop of SlowFade antifade reagent (Molecular Probes) was dispensed on the glass slide, covered with cover slip, and imaged

PLoS ONE Alerts: New Articles Mariana M. Hecht et al. 2010

Slides were mounted with ProLong Gold antifade reagent (Invitrogen).

PLoS ONE Alerts: New Articles Tsufit Gonen-Gross et al. 2010

Immunostained slides were cover-mounted with Prolong Gold antifade reagent (Invitrogen).

PLoS ONE Alerts: New Articles Jamila Khallou-Laschet et al. 2010

The dishes were washed twice with PBS, and then 2 ml of PBS supplemented with 2. 5\% (w / v) DABCO as an antifade was added to the dishes.

PLoS Biology: New Articles 2009

2O, mounted on glass slides with the ProLong® Gold antifade reagent (Molecular Probes) and viewed on a BioRad Radiance 2000 Scanning Laser Confocal Microscope (CLSM).

PLoS ONE Alerts: New Articles Cira Daniela Rinaudo et al. 2010

2O, mounted on glass slides with the ProLong® Gold antifade reagent (Molecular Probes) and viewed on a BioRad Radiance 2000 Scanning Laser Confocal Microscope (CLSM).

PLoS ONE Alerts: New Articles Cira Daniela Rinaudo et al. 2010

Coverslips were mounted using ProLong® Gold antifade reagent with DAPI (Invitrogen, Cat. no.

PLoS ONE Alerts: New Articles Malgorzata Romanowska et al. 2010