Definitions

from The American Heritage® Dictionary of the English Language, 4th Edition

  • abbr. complementary DNA

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • complementary DNA
  • copy DNA

Etymologies

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Examples

  • Reverse-Transcription P - dCTP 33 radio-labelled cDNA probes Hybridization to microarray filters Use Phosphor Imager laser scanner to obtain densities of each spot on filter.

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  • In 1992, the cDNA of GFP was cloned by Dr. Douglas Prasher, who was then at Woods Hole Oceanographic Institution.

    Osamu Shimomura - Autobiography

  • At that time, however, it was commonly believed that expressing the cDNA in living organisms would not produce fluorescent GFP, because the formation of its chromophore requires the reactions of condensation and dehydrogenation that are not expected to occur spontaneously.

    Osamu Shimomura - Autobiography

  • The cDNA of aequorin was cloned and recombinant aequorin was made in 1985, but the patent owners of recombinant aequorin, the University of Georgia and Chisso Corporation of Japan, were not sure about expanding the use of aequorin, thus the general use of recombinant aequorin was delayed.

    Osamu Shimomura - Autobiography

  • In 1994, however, Dr. Martin Chalfie of Columbia University tried to express the cDNA in E. coli and a nematode worm, and he and his colleagues unexpectedly observed the fluorescence of the expressed GFP.

    Osamu Shimomura - Autobiography

  • We will also pursue further studies delineating immediate early gene/cytokine/chemokine expression in human partial liver grafts utilizing cDNA microarrays on liver biopsies obtained after reperfusion, in both cadaveric and living donor partial grafts.

    Basic Biliary Atresia Research

  • Early gene expression in the liver graft after reperfusion - use of cDNA microarray, RNA protection assays, and multimeric RT-PCR technology.

    Basic Biliary Atresia Research

  • And what came up was Douglas Prasher's paper about the cloning of the cDNA for green fluorescent protein.

    Martin Chalfie - Interview

  • A further 4%-5% of reads map to unannotated genomic regions that can be aligned to spliced ESTs and mRNAs from high-throughput cDNA sequencing efforts, and only 2.2%-2.5% of reads cannot be explained by any of the aforementioned categories.

    Junk DNA is still junk - The Panda's Thumb

  • These include opiate and antimicrobial peptides from amphibian skin, neuropeptides from snail ganglia, a hormone from crustaceans, and a constituent of a spider venom. cDNA cloning has shown that at those positions where a d-amino acid is found in the end-product, a normal codon for the corresponding l-amino acid is present.

    Always finish what you start…

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