calpeptin

Application of calpeptin and calpain inhibitor III did not affect α-fodrin fragmentation in primary term trophoblasts and forskolin-treated BeWo cells, but decreased secretion of

Naturejobs - All Jobs M Gauster 2010

(A) Representative immunoblots showing protein levels of NR1, NR2A, and NR2B subunits in slices treated with 10 µM UCB for 48 h in the absence or presence of calpain inhibitors, leupeptin (100 µM) or calpeptin (100 µM).

PLoS ONE Alerts: New Articles Fang-Yu Chang et al. 2009

(F) Summary of experiments showing the induction of LFS-induced LTD in slices simultaneously treated with 10 µM UCB with calpeptin (100 µM) for 24 or 48 h.

PLoS ONE Alerts: New Articles Fang-Yu Chang et al. 2009

D-APV, nimodipine, leupeptine, and calpeptin were purchased from Tocris Cookson (Bristol, UK).

PLoS ONE Alerts: New Articles Fang-Yu Chang et al. 2009

To examine the functional consequences of the blockade of calpain activity on the UCB effects, slices were co-exposure to UCB (10 µM) and leupeptin or calpeptin.

PLoS ONE Alerts: New Articles Fang-Yu Chang et al. 2009

(E) Summary of experiments showing the induction of HFS-induced LTP in slices simultaneously treated with 10 µM UCB with calpeptin (100 µM) for 24 or 48 h.

PLoS ONE Alerts: New Articles Fang-Yu Chang et al. 2009

Figure 10A-D, both leupeptin (100 µM) and calpeptin (100 µM) completely prevented UCB-induced down-regulation of NR2A and NR2B subunit proteins.

PLoS ONE Alerts: New Articles Fang-Yu Chang et al. 2009

h on the basal calpain activity in the absence or presence D-APV (50 µM), nimodipine (20 µM), leupeptin (100 µM), and calpeptin (100 µM).

PLoS ONE Alerts: New Articles Fang-Yu Chang et al. 2009

UCB-induced decrease in NR1 subunit was not affected by addition of either leupeptin or calpeptin (

PLoS ONE Alerts: New Articles Fang-Yu Chang et al. 2009

In parallel experiments, we also found that calpeptin completely abolished UCB-induced impairment of LTP (calpeptin-control: 142. 5±5.6% of baseline, n = 10; calpeptin-UCB 10 µM for 24 h: 135. 2±6.7% of baseline, n = 8; calpeptin-UCB

PLoS ONE Alerts: New Articles Fang-Yu Chang et al. 2009