cap-binding

Although they had suspected that the nucleotides might physically block Argonaute's cap-binding site, instead they found that adding nucleotides to the mix actually enhanced Argonaute's ability to bind to an mRNA cap.

HHMI News 2010

Interestingly, most of the ribosomal proteins found in our study belong to the small, 40S ribosomal subunit, which is known to associate with another multiprotein factor that we have identified, the cap-binding complex eIF4F

PLoS ONE Alerts: New Articles Vincent Gache et al. 2010

Although they had suspected that the nucleotides might physically block Argonaute's cap-binding site, instead they found that adding nucleotides to the mix actually enhanced Argonaute's ability to bind to an mRNA cap.

HHMI News 2010

For preparative purification of cap-binding complexes the reaction described above was scaled up 4-fold and proteins detected by coomassie staining were analyzed at the University of Massachusetts Medical School Proteomics Facility (Worcester, MA).

PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009

Since gemin5 is also capable of cap-binding, it may conceivably participate in RNP complexes containing mRNAs.

PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009

Buffers used for lysate preparation (PLB) and binding reactions (NT2) were identical to those used in cap-binding assays.

PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009

Gingras AC, Marcotrigiano J, Favre D, et al. (1998) Cloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding protein.

PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009

One possibility is that gemin5 binds to the prototype cap-binding protein, eIF4E.

PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009

The WD repeat domains of gemin5 are required for association with cap-binding complexes

PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009

Mathonnet G, Fabian MR, Svitkin YV, Parsyan A, Huck L, et al. (2007) MicroRNA inhibition of translation initiation in vitro by targeting the cap-binding complex eIF4F.

PLoS ONE Alerts: New Articles Shelton S. Bradrick et al. 2009