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- n. A fluorescent dye that is used as a biological tracer
Sorry, no etymologies found.
Briefly, splenocytes were labeled with 5 µM carboxyfluorescein diacetate succinimidyl ester (CFSE, Molecular Probes, Invitrogen) at day 0 and then stimulated with anti-CD3 / CD28 antibodies plus IL-2 during 72 hours in isotonic or hypertonic conditions.
We assessed cell proliferation by labeling cells with carboxyfluorescein diacetate succinimidyl ester and quantifying the fluorescence by FACS analysis.
N-hydroxysuccinimide (NHS) - Fluorescein, [5 - (and 6) - carboxyfluorescein succinimidyl ester] (Pierce Biotechnology Inc, Rockport, IL) in parallel with CPMV-WT. 200 µg of virus at a concentration of 1 mg / ml in 10\% DMSO was mixed with various amounts of excess NHS-Fluorescein relative to viral asymmetric subunits (60 / particle) in the following ratios: 1, 5, 10, 25, 50,
EAE or control rats were injected intra-CSF with bone marrow-derived myeloid DCs labeled with the fluorescent marker carboxyfluorescein diacetate succinimidyl ester (CFSE).
DCs were labeled with CFSE (carboxyfluorescein diacetate succinimidyl ester, Molecular Probes) as previously described
Target cells were labeled with 0.5 µM carboxyfluorescein diacetate succimidyl ester (CFSE; Molecular Probes Europe,
Two complementary experimental procedures were followed: i) in a first set of experiments, we tracked endogenous CSF-circulating antigen-presenting cells (APCs) by injecting fluorescent microspheres into the CSF of rats induced for EAE; ii) in a second set of experiments, bone marrow-derived myeloid DCs were labeled with the fluorescent marker carboxyfluorescein diacetate succinimidyl ester (CFSE) then injected into the CSF of EAE rats, at the clinical peak of the disease, or control rats.