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- n. caltractin
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(C) HeLa cells stably expressing a centrin-GFP fusion (green) were either fixed and stained for endogenous βarr2 using either rARR or gBARR2 polyclonal antibodies (red) or transfected with plasmids encoding for the βarr2-Cherry fusion (red), then fixed and directly observed.
To more precisely characterize βarr2 localization at the centrosome, we used centrin as a marker of the distal part of centrioles
This specific localization was further confirmed with 3D reconstruction of deconvoluted images, in which juxtaposition of βarr2 and centrin was clearly visible (