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Examples
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IX, is also the most complex and least well understood step of chlorophyll synthesis, as magnesium chelatase employs multiple protein subunits (three in bacteria, four in plants / algae) in varying stoichiometric ratios.
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Magnesium chelatase catalyzes the first step of chlorophyll synthesis in plants, algae, and photosynthetic bacteria.
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In this Paper of the Week, Artur Sawicki and Robert Willows methodically address the relevant kinetic questions of this enzyme using individual subunits of Rhodobacter capsulatus magnesium chelatase (BchD, BchH, and BchI); they treat one portion of the active unit as the enzyme while treating other subunits of the active complex as substrates.
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ATP hydrolysis plays multiple roles in catalysis and regulation of magnesium chelatase activity by causing a series of conformational Kinetic properties of two magnesium chelatase subunits, revealing Michaelis-Menten kinetics with BchI and sigmoidal kinetics with BchH.
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The mau cluster is flanked by mobile elements, but the dcm cluster disrupts a gene annotated as chelatase and for which we propose the name "island integration determinant" (
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