Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- verb Simple past tense and past participle of
citrate .
Etymologies
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Examples
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With a sterile 10 c.c. graduated pipette transfer 1 c.c. citrated blood from the Erlenmeyer flask to each tube of liquefied agar.
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China green agar (Werbitski), 207 citrated blood agar, 171
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When preparing citrated blood agar (page 171) it is always advisable to pour several blood agar tubes into plates, which can be stored in the ice chest ready for use at any moment for surface plate cultures.
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Measure out 2 c.c. citrated human blood (collected at a surgical operation or a venesection, or withdrawn by venipuncture from the median basilic or median cephalic vein of a normal adult) into a centrifuge tube and centrifugalise thoroughly.
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Remove the needle from the nozzle of the syringe and deliver the citrated blood into a small Ehlenmeyer flask containing about 250 c.c. of nutrient broth.
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Rotate the tube between the hands in order to diffuse the citrated blood evenly throughout the agar.
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This process consists in simply passing over the ammonio-citrated paper on which such a latent picture has been impressed,
Photographic Reproduction Processes Peter C. Duchochois
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For flowcytometric analysis, CD3+ were kept on ice and washed extensively with citrated PBS containing 1% FCS. 10 µl of FITC, APC or PE - conjugated antibody was added and incubated on ice for 20 min. 300 µl FACS buffer was added and T cells were pelleted, resuspended in 200 µl buffer.
PLoS ONE Alerts: New Articles Timothy R. D. J. Radstake et al. 2009
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