cotransfection love

Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. The simultaneous transfection with two, unrelated nucleic acids, one of which carries a marker gene

Etymologies

Sorry, no etymologies found.

Examples

  • DNA-cotransfection was performed in MC3T3-E1 cells (ATCC, #CRL-2593) using calcium phosphate transfection, and the activities of Luciferase and ß-Galactosidase were measured two days later as described For the generation of Col1a1-Krm2 transgenic mice the ORF encoding Krm2 was placed under the control of an osteoblast-specific 2. 3kb

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  • The cotransfection results of each BmNPV genomic library plasmid, hr3 plasmid and reporter plasmid showed that there were eight library plasmids stimulated the luciferase gene expression remarkably.

    BioMed Central - Latest articles

  • Although overexpression of wild-type p53 also downregulates TCF-4 in BE1 cells, cotransfection of p53 and Axin-Delta-beta-ca did not downregulate TCF-4 further.

    BioMed Central - Latest articles

  • Validation of the reporter was performed by cotransfection in U2OS cells under screening conditions.

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  • Our results from in vitro nuclear import assays with permeabilized cells and in vivo cotransfection experiments reveal that importin 13 facilitates nuclear import of both histone fold heterodimers.

    Journal of Biological Chemistry current issue

  • Here we report that the NM23-H1 interacts with MIF in cells, as demonstrated by cotransfection and coimmunoprecipitation experiments.

    Journal of Biological Chemistry current issue

  • We then employed a cDNA library consisting of approximately 15,000 arrayed full-length human cDNAs to identify activators of this reporter by transient cotransfection in U2OS osteosarcoma cells (

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  • This construct was validated by cotransfection with the known INK4a activator ETS2, which resulted in 4-5 fold activation of the reporter (

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