Definitions
from The American Heritage® Dictionary of the English Language, 5th Edition.
- noun A stain of a contrasting color used to color the components in a microscopic specimen that are not made visible by the principal stain.
- transitive verb To color (a microscopic specimen) with such a stain.
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun microscopy A
stain contrasting with the principal stain in color, thus making the stainedstructure easier to see - verb microscopy To stain with a counterstain
from WordNet 3.0 Copyright 2006 by Princeton University. All rights reserved.
- noun a stain of contrasting color that is used when the principal stain does not show the structure clearly
Etymologies
from Wiktionary, Creative Commons Attribution/Share-Alike License
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Examples
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Stain section of kidney tissue provided (showing abscess formation by Staphylococcus aureus) by Gram's method, and counterstain with cosin.
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Following PBS wash, slides were counterstained with a Hoescht counterstain (Sigma B2883).
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Bottom: reelin immunofluorescence (red) overlaid with MAP2 counterstain
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Anti-calbindin antibody labels Purkinje cell bodies and dendritic arbors, while the Nissl counterstain (purple) labels all neuronal cell bodies.
PLoS ONE Alerts: New Articles Cristin D. Davidson et al. 2009
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Detection was completed using HRP-mediated hydrogen peroxide immunostaining (AEC+, DAKO), with hematoxylin as a counterstain.
PLoS ONE Alerts: New Articles Nicholas J. Haley et al. 2009
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Following incubation with fluorescence-conjugated secondary antibody (at room temperature for 60 min) and rinses, slides were incubated with DAPI (1: 10,000, Sigma, St. Louis, MO) for 15 minutes to counterstain nuclei, mounted, and cover-slipped.
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Acridine orange (Citrus sinensis) fluorescent counterstain auramine acid-fast stain
xml's Blinklist.com 2008
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α (brown; g; nuclear counterstain is methyl green), Ttf1
Naturejobs - All Jobs Harald C Ott 2010
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