from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun biology The
freezingof a specimen by immersionin liquid nitrogen
from Wiktionary, Creative Commons Attribution/Share-Alike License
As a consequence, the time spent between the last LM image and cryofixation of cells was longer (8 seconds) than what can be achieved without this step (5 seconds).
Two options have been used to date: chemical fixation and cryofixation.
This method uses laser pre-micro-patterned aclar discs as culture substrates, that are compatible with cryofixation, followed by resin embedding.