Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License.

  • verb Simple past tense and past participle of cryoprotect.
  • adjective protected from cold by the action of a cryoprotectant

Etymologies

Sorry, no etymologies found.

Examples

  • Brains were removed and cryoprotected by soaking in 30% sucrose solution in phosphate buffer until they sank.

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  • The crystals were cryoprotected in the mother liquor plus the addition of 15% glycerol and flash frozen in a nitrogen stream at − 180°C.

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  • The images shown here were taken from cryoprotected cells, as the freezing quality was improved.

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  • Brains were removed, post-fixed overnight in perfusant, then cryoprotected in a 30% sucrose solution.

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  • The crystals were cryoprotected in the mother liquor plus the addition of 15% glycerol and flash frozen in a nitrogen stream at − 180°C.

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  • Briefly, the fixed samples were cryoprotected with PVP/sucrose (20% polyvinyl pyrrolidone [Sigma] in 2.3 M sucrose) overnight and frozen in liquid nitrogen before being cut into thin sections with Leica Ultracut UCT with EMFCS attachment (Leica Microsystems Inc.).

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  • Our results indicate that small numbers of organ cells had withstood and / or evaded cryoinjury from ice crystals and / or osmotic stress within the organ and that nuclei in the cells were intact after defrosting the non-cryoprotected frozen organs.

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  • There have been attempts to rescue animal genetic resources from dead cells or non-cryoprotected frozen specimens.

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  • Briefly, animals were perfused with 4\% paraformaldehyde, the brains removed from the skulls, cryoprotected and sectioned at 40 µm on a dry ice-cooled sliding microtome.

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  • To our knowledge, this is the first report of the resurrection of a dead elite livestock specimen from a non-cryoprotected frozen organ by cloning.

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