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centrifugationusing a cytocentrifuge
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Total leukocyte count was determined with a hemacytometer, and differential count by cytocentrifugation of 300 µl of bronchoalveolar lavage fluid at 450×g for 5 min, followed by Wright-Giemsa staining.
Cells were immediately fixed in 1\% paraformaldehyde and distributed on gelatinized glass slides by cytocentrifugation at a density of 5×10