Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • abbr. deoxyuridine triphosphate

Etymologies

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Examples

  • Colonic injury was evaluated by assessing colon length, colonocyte apoptosis (by terminal dUTP nick-end labeling), colonic cytokine production and histological score.

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  • Apoptotic changes were further evaluated by a variety of techniques, including the terminal deoxynucleotidyl transferase (TdT) - mediated dUTP

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  • A total of 20 µg of RNA was transcribed to DNA and labeled with aminoallyl dUTP using reverse transcriptase (Superscript III; Invitrogen) and random hexamers (TAKARA Bio) as primers.

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  • Then 5 µl of 10×dNTP mix were added as well as 3 µl of 1 mM dUTP-Cy3 or dUTP-Cy5 (GE Healthcare) and 40 U of Klenow fragment (Invitrogen).

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  • + (Fenton reaction) for 5 min. Apoptosis rate was determined by Annexin V labelling and terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling assay.

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  • Apoptosis was confirmed by DNA fragmenatation, western blot and terminal transferase-mediated dUTP-digoxigenin-end labeling (TUNEL) assay.

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  • Apoptotic cells were detected in situ by performing TUNEL (TdT-mediated digoxigenin labeled dUTP nick end labeling) staining.

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  • Abbreviations: TPA, 12-O-tetradecanoylphorbol-13-acetate • TUNEL, terminal deoxynucleotidyltransferase dUTP nick end labeling • HME, human mammary epithelial • siRNA, small interfering RNA • PBS, phosphate-buffered saline • CHAPS,

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  • TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick end-labeling) - positive apoptotic cells can be observed throughout the head and trunk and in the ventral wall of the dorsal aorta (VW-DA) where the first definitive hematopoietic stem cells presumably emerge at 28 hpf

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  • T. parva-infected cell line by reverse transcription followed by in vitro transcription with simultaneous incorporation of amino-allyl dUTP using the Aminoallyl Message Amp RNA amplification kit (Ambion Inc.,

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