Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun biochemistry The removal of the
sugar entity from aglycogen (but especially from aglycoprotein )
Etymologies
Sorry, no etymologies found.
Support
Help support Wordnik (and make this page ad-free) by adopting the word deglycosylation.
Examples
-
Lysates from rat retinal ganglion RGC5 cells were subjected to N - or O-deglycosylation or membrane protein extraction.
PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010
-
Lysates from RGC5 cells were treated with peptide N-glycosidase F (PNGase F, for N-deglycosylation) or O-glycosidase (for O-deglycosylaton) without or with α-2 (3,6,8,9) - neuraminidase (sialidase), and Western blotted for optineurin.
PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010
-
Cross-contamination of cytosolic proteins into the membrane fraction is typically Total protein (50-100 µg) from RGC5 cells or those transfected with pTarget-FLAG-OPTN was digested with PGNase F (0.25 units, Enzymatic In-Solution N-deglycosylation kit, Sigma) for N-deglycosylation or with O-glycosidase (2.5 mUnits, Sigma) without or with addition of sialidase A (5 mUnits, Sigma) for O-deglycosylation per manufacturer's instructions.
PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010
-
Lysates from rat retinal ganglion RGC5 cells were subjected to N - or O-deglycosylation or membrane protein extraction.
PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010
-
Lysates from rat retinal ganglion RGC5 cells were subjected to N - or O-deglycosylation or membrane protein extraction.
PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010
-
The purified RNase B (Sigma) was used as a positive control in parallel in the N-deglycosylation experiment and the purified bovine fetuin (Sigma) as a positive control in the O-deglycosylation experiment.
PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010
-
The deglycosylation experiments with PNGase F (Sigma) were carried out according to the manufacturer's instructions.
PLoS ONE Alerts: New Articles Chang-Jin Park et al. 2010
-
The deglycosylation experiments with PNGase F (Sigma) were carried out according to the manufacturer's instructions.
PLoS ONE Alerts: New Articles Chang-Jin Park et al. 2010
-
Lysates from rat retinal ganglion RGC5 cells were subjected to N - or O-deglycosylation or membrane protein extraction.
Elites TV PLoS ONE 2010
-
Lysates from RGC5 cells were treated with peptide N-glycosidase F (PNGase F, for N-deglycosylation) or O-glycosidase (for O-deglycosylaton) without or with α-2 (3,6,8,9) - neuraminidase (sialidase), and Western blotted for optineurin.
PLoS ONE Alerts: New Articles Hongyu Ying et al. 2010
Comments
Log in or sign up to get involved in the conversation. It's quick and easy.