Definitions
from Wiktionary, Creative Commons Attribution/Share-Alike License.
- noun biochemistry, especially in combination The univalent radical derived from a
deoxynucleotide
Etymologies
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Examples
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Apoptotic changes were further evaluated by a variety of techniques, including the terminal deoxynucleotidyl transferase (TdT) - mediated dUTP
PLoS ONE Alerts: New Articles Tai-Ho Hung et al. 2010
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The resulting cDNA was subjected to a tailing reaction with dCTP using terminal deoxynucleotidyl transferase, and then was PCR amplified using same L gene specific primer and an anchored oligo dG primer.
PLoS ONE Alerts: New Articles Arthur S. Samuel et al. 2010
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Cells were then washed with PBS prior to incubation with recombinant terminal deoxynucleotidyl transferase (TdT) and biotinylated nucleotides for 1 h at 37°C, which incorporates the biotinylated nucleotides at 3′ ends of fragmented DNA.
PLoS ONE Alerts: New Articles Maria Marchetti et al. 2009
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D-GalN evoked cytosolic Bax and mitochondrial cytochrome C translocation and activated proinflammatory nuclear factor-kappa B (NF-kB) and activator protein-1 (AP-1) translocation, contributing to the increase of intercellular adhesion molecule-1 expression, terminal deoxynucleotidyl transferase-mediated nick-end labeling
BioMed Central - Latest articles Chia-Jung Yu Bor-Ru Lin 2009
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Tumor cells are positively stained for Tdt (terminal deoxynucleotidyl transferase) and (D)
PLoS ONE Alerts: New Articles Silke H. Raffegerst et al. 2009
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A hallmark of B cell development during fetal life is the lack of expression of deoxynucleotidyl transferase which prevents non-templated nucleotide additions in the V-D and D-J junction of the BCR heavy chain.
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A poly-G tail was added to the cDNA 3′ end using terminal deoxynucleotidyl transferase (TDT) (Promega) by incubating the purified cDNA with 1× TDT buffer (Promega), 0.83 mM dGTP and 20 U of enzyme at 37°C for 1 h.
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Sections treated without terminal deoxynucleotidyl transferase served as negative control.
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+ (Fenton reaction) for 5 min. Apoptosis rate was determined by Annexin V labelling and terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling assay.
Naturejobs - All Jobs A E Gawad 2009
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TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick end-labeling) - positive apoptotic cells can be observed throughout the head and trunk and in the ventral wall of the dorsal aorta (VW-DA) where the first definitive hematopoietic stem cells presumably emerge at 28 hpf
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