dideoxy

The work culminated in the development of the "dideoxy" technique for DNA sequencing around

Frederick Sanger - Autobiography 1981

The work culminated in the development of the "dideoxy" technique for DNA sequencing around

Frederick Sanger - Biography 1964

Amplified fragments were cloned into the pGEM-T Easy vector (Promega) and sequenced in both directions with dideoxy terminators (ABI BigDye Kit).

PLoS ONE Alerts: New Articles Hong Zhang et al. 2010

In this sense, dideoxy-sequencing served its purpose.

PLoS ONE Alerts: New Articles Vassiliki Kotoula et al. 2009

Overall, caution is warranted when performing validation trials with dideoxy-sequencing as the golden standard to determine the specificity of methods with higher mutation detection efficiency on FFPE-DNA templates.

PLoS ONE Alerts: New Articles Vassiliki Kotoula et al. 2009

Where Venter's genome was sequenced with Sanger dideoxy sequencing, the gold standard technology of the day; Watson's genome was sequenced with the first of the Next Generation Sequencing instruments, the 454 (now owned by Roche).

ScienceBlogs Channel : Life Science 2009

In comparison to dideoxy-sequencing, pyrosequencing seems to be much more efficient in analyzing 60-80 nt long sequences from FFPE-DNA templates.

PLoS ONE Alerts: New Articles Vassiliki Kotoula et al. 2009

Tumor FFPE-DNA from 135 diagnostic and 75 low-quality control samples was obtained upon macrodissection, tested for fragmentation and assessed for KRAS mutations with dideoxy-sequencing and with two Q-PCR methods (Taqman-minor-groove-binder [TMGB] probes and DxS-KRAS-IVD).

PLoS ONE Alerts: New Articles Vassiliki Kotoula et al. 2009

At present, although Q-PCR methods are in general more efficient than dideoxy-sequencing on FFPE material, since they target small sequences likely to be preserved upon formalin fixation, it is still not clear how they perform on a daily routine basis in this context.

PLoS ONE Alerts: New Articles Vassiliki Kotoula et al. 2009

Herein, we report on our experience on prospective diagnostic KRAS mutation testing with three methods including dideoxy-sequencing that was used as the reference method for mutation validation, and two Q-PCR methods, the IVD DxS-KRAS test and a Taqman-minor-groove-binder (Taqman-MGB, TMGB) test that was standardized and validated in our lab, by taking into consideration not only the efficiency of the methods but mostly focusing on template characteristics.

PLoS ONE Alerts: New Articles Vassiliki Kotoula et al. 2009