dithiothreitol

Also, by shifting the Tamm-Horsfall protein to the high-speed pellet, the use of dithiothreitol makes it feasible to use Tamm-Horsfall protein to normalize excretion rates of exosomal proteins in spot urines.

Naturejobs - All Jobs Patricia Fernández-Llama 2010

Here we show that addition of dithiothreitol to the low-speed pellet disrupted the polymeric network, presumably by reduction of disulfide bonds linking the monomers.

Naturejobs - All Jobs Patricia Fernández-Llama 2010

Here we show that addition of dithiothreitol to the low-speed pellet disrupted the polymeric network, presumably by reduction of disulfide bonds linking the monomers.

Naturejobs - All Jobs Patricia Fernández-Llama 2010

Samples were subjected to reduction with dithiothreitol, alkylation with iodoacetamide, and in-gel digestion with trypsin or chymotrypsin overnight at pH 8.3, followed by reversed-phase microcapillary/tandem mass spectrometry (LC/MS/MS).

PLoS ONE Alerts: New Articles Dana M. Gwinn et al. 2010

CBB, Coomassie brilliant blue R-250; DTT, dithiothreitol; 1D-PAGE, one-dimensional polyacrylamide gel electrophoresis; 2D-PAGE, two-dimensional polyacrylamide gel electrophoresis; ECM, extracellular matrix; GlcNAc,

Naturejobs - All Jobs Noriko Takahashi 2010

Also, by shifting the Tamm-Horsfall protein to the high-speed pellet, the use of dithiothreitol makes it feasible to use Tamm-Horsfall protein to normalize excretion rates of exosomal proteins in spot urines.

Naturejobs - All Jobs Patricia Fernández-Llama 2010

Alternatively, immunoprecipitations were subjected to in vitro phosphorylation by resuspension in kinase buffer (50 mM Tris-HCL pH7. 5, 10 mM MgCl2, 1 mM EGTA, 2 mM DTT, 2 mM dithiothreitol, 0. 01% BRIJ35, and 150 mM NaCl2), followed by addition of

PLoS Biology: New Articles Marcel A. T. M. van Vugt et al. 2010

Figure 4A also shows that the reducing reagent dithiothreitol

PLoS ONE Alerts: New Articles Akihiko Ozawa et al. 2009

Cells were washed once with PBS and resuspended in ice-cold sucrose buffer (250 mM sucrose, 0.5 mM EGTA, 20 mM Hepes-K, pH 7.0) containing 2 mM dithiothreitol.

PLoS ONE Alerts: New Articles 2009

Figure 4A also shows that the reducing reagent dithiothreitol

PLoS ONE Alerts: New Articles 2009