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- n. The emission of light as a result of an electrochemical reaction
Thyroid-stimulating hormone (TSH) was measured with electrochemiluminescence immuno assay
Voltage is applied and resulting electrochemiluminescence is measured.
Electrochemiluminescence technology utilizes a stable ruthenium metal chelate (Ori-TAG) which, in the presence of tripropylamine (TPA), generates electrochemiluminescence upon voltage application.
The secondary antibodies, anti-rabbit HRP and anti-mouse HRP (Sigma), were used at 1: 10,000 and detection of signal utilized Thermo Scientific electrochemiluminescence (ECL) reagents.
In each study, samples were first screened using an electrochemiluminescence (ECL) assay.
The patient flowchart and the protocol for this trail is available as supporting information; see Plasma levels of chemokines and vascular cytokines were assessed by MesoScale technology, which is a multiplex type of ELISA technology that relies on electrochemiluminescence, according to the manufacturer's protocol.