Definitions

from Wiktionary, Creative Commons Attribution/Share-Alike License

  • n. Plural form of eluate.

Etymologies

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Examples

  • The elution was repeated with new elution buffer, and the eluates pooled.

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  • Strikingly, although TCF-4 protein could be detected in eluates after incubation with HD or CD

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  • Refolding of falcipain-2 was achieved by diluting the eluates 100-fold in 100 mM Tris-HCl, 30\% glycerol, 250 mM arginine, 1 mM EDTA, 1 mM reduced-glutathione (GSH), 1 mM oxidized-glutathione (GSSG), pH 9.2 and incubating at 4°C overnight.

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  • Refolding of falcipain-3 was achieved by diluting the eluates 100-fold in 100 mM Tris-HCl, 15\% sucrose, 250 mM arginine, 1 mM EDTA, 1 mM GSH, 1 mM GSSG, pH 9.2 and incubating at 4°C overnight.

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  • The eluates were evaporated to approximately 20 µl using air and then reconstituted in acetonitrile in water (1: 1), containing 0. 1% formic acid, to a final extract volume of 1.0 ml.

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  • Lanes 1, 5: cell extract; lanes 2, 6: supernatant after incubation of extracts with antibody-coupled protein G or protein A sepharose beads; lanes 3, 7: RNP eluates after treatment of beads with SDS-EDTA; lanes 4, 8:

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  • RNP eluates probed with the alternate PUM antibody. 25 µg (PUM1) or 50 µg (PUM2) of extracts and supernatants, 5\% of captured beads and 1\% of eluates were loaded.

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  • We found approximately 300 proteins in the column eluates from two columns with peptide antibodies, shown in

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  • Formaldehyde crosslinks were reversed by incubating eluates (including

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